Medium data

Search for medium no. = [399].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

399   DESULFOVIBRIO MAGNETICUS MEDIUM

K2HPO4  0.2     g
NH4Cl  0.06    g
Fe(III) quinate solution (see below)  2.0     ml
Trace minerals (see Medium No. 151)  4.0     ml
Trace vitamins (see Medium No. 197)  8.0     ml
Sodium fumarate  0.58    g
Sodium pyruvate  0.44    g
L-Cysteine·HCl·H2O  0.05    g
Distilled water  1.0     L
Adjust pH to 7.0. Prepare medium anaerobically under a N2 gas atmosphere.
Fe(III) quinate solution:
FeCl3·6H2O  0.45    g
Quinic acid  0.19    g
Distilled water  100.0     ml