Medium data
Search for medium no. = [389].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
389 DESULFOVIBRIO MEDIUM
| K2HPO4 | 0.5 | g |
| NH4Cl | 1.0 | g |
| Na2SO4 | 1.0 | g |
| MgSO4·7H2O | 2.0 | g |
| CaCl2·2H2O | 0.1 | g |
| Sodium lactate | 2.0 | g |
| Yeast extract | 1.0 | g |
| FeSO4·7H2O | 0.5 | g |
| Sodium thioglycolate | 0.1 | g |
| Ascorbic acid | 0.1 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 1.0 | L |
Mix ingredients, except FeSO4·7H2O, sodium thioglycolate and ascorbic acid, bring to a boil, and cool down to room temperature under a N2 gas stream. Add FeSO4·7H2O in 10 ml distilled water, and sodium thioglycolate and ascorbic acid in 10 ml distilled water, and adjust pH to 7.8 with NaOH. While swirling the medium, dispense into culture vessels under a N2 gas stream, seal with butyl rubber stoppers, and autoclave. Adjust pH to 6.8-7.0, if necessary.
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