Medium data

Search for medium no. = [377].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

377   LENTIBACILLUS MEDIUM

Casamino acids (BD-Difco)  5.0     g
Yeast extract (BD-Difco)  5.0     g
Sodium glutamate  1.0     g
Trisodium citrate  3.0     g
MgSO4·7H2O  20.0     g
KCl  2.0     g
NaCl  100.0     g
FeCl2·4H2O  36.0     mg
MnCl2·4H2O  0.36    mg
Add components to distilled water and bring volume to 1.0 L.
Adjust pH to 7.0 - 7.2.
For preparation of solid medium, add 20.0 g/L agar.