Medium data
Search for medium no. = [360].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
360 MMJ MEDIUM
| NaCl | 30.0 | g |
| K2HPO4 | 0.14 | g |
| CaCl2·2H2O | 1.0 | g |
| NaNO3 | 0.8 | g |
| MgSO4·7H2O | 3.4 | g |
| MgCl2·6H2O | 4.18 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 5.0 | mg |
| Na2SeO3·5H2O | 5.0 | mg |
| Fe(SO4)3·xH2O | 0.05 | g |
| H2WO4 | 5.0 | mg |
| Trace minerals (see Medium No. 324) | 10.0 | ml |
| NaHCO3 | 1.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients, except NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O, adjust pH to 7.5 - 8.0, and autoclave. Separately autoclave NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O solutions (5% each) under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O solutions aseptically and anaerobically. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).
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