Medium data

Search for medium no. = [360].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  30.0     g
K2HPO4  0.14    g
CaCl2·2H2O  1.0     g
NaNO3  0.8     g
MgSO4·7H2O  3.4     g
MgCl2·6H2O  4.18    g
KCl  0.33    g
NiCl2·6H2O  5.0     mg
Na2SeO3·5H2O  5.0     mg
Fe(SO4)3·xH2O  0.05    g
H2WO4  5.0     mg
Trace minerals (see Medium No. 324)  10.0     ml
NaHCO3  1.0     g
L-Cysteine·HCl·H2O  0.5     g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients, except NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O, adjust pH to 7.5 - 8.0, and autoclave. Separately autoclave NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O solutions (5% each) under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O solutions aseptically and anaerobically. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).

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