Medium data

Search for medium no. = [333].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

333 POTATO-SUCROSE AGAR

Potato	`200.0`	g
Sucrose	`20.0`	g
Agar	`20.0`	g
Distilled water	`1.0`	L

Adjust pH to 5.6.
Wash potatoes with tap water, peel and cut into 1 cm cubes. Rinse with tap water quickly and boil 200 g of potato cubes with 1.0 L of distilled water for 20 min. Mash and squeeze through a muslin bag. Add agar and boil until melting. Add sucrose and mix thoroughly. Make up to 1.0 L, adjust pH and autoclave for sterilization.