Medium data

Search for medium no. = [327].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Bacto peptone (BD-Difco)  10.0     g
Yeast extract (BD-Difco)  0.5     g
L-Cysteine·HCl·H2O  1.0     g
Agar  15.0     g
Distilled water  800.0     ml
Fetal bovine serum  200.0     ml
Dissolve peptone, yeast extract and cysteine in 800 ml of distilled water. Adjust pH to 6.5 with NaOH. Add agar and dissolve by heating. After autoclaving, cool to 45C and add serum.