Medium data

Search for medium no. = [322].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

322   THERMOVIBRIO RUBRUM MEDIUM

Synthetic seawater (2 x, see Medium No. 244)  500.0     ml
KH2PO4  0.5     g
(NH4)2SO4  0.25    g
KNO3  1.0     g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Distilled water  500.0     ml
Mix ingredients, except KNO3 and Na2S·9H2O, adjust pH to 6.5 - 7.0 with H2SO4, and autoclave under a N2 atmosphere. Separately autoclave 10% KNO3 solution under a N2 atmosphere. Neutralize 5% Na2S·9H2O solution, and autoclave under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile KNO3 and Na2S·9H2O solutions, and readjust pH, if necessary. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v) gas mixture.