Medium data

Search for medium no. = [306].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

306   HALOALKALIPHILE MEDIUM

Yeast extract (BD-Difco)  2.0     g
Casamino acids (BD-Difco)  2.0     g
Sodium glutamate  1.0     g
Trisodium citrate  3.0     g
MgSO4·7H2O  0.1     g
CaSO4·2H2O  0.1     g
KCl  1.0     g
NaCl  200.0     g
Na2CO3  8.0     g
Add components except Na2CO3 to distilled water and bring volume to 900 ml. Adjust pH to 6.5 and autoclave. Dissolve Na2CO3 in 100 ml distilled water and autoclave separately. Aseptically add the sterile Na2CO3 solution to the medium. Check final pH to be 9.0 - 9.5.