303 PE MEDIUM
Basal salts solution (see below) | 5.0 | ml |
Phosphate solution (see below) | 5.0 | ml |
Vitamin solution (see below) | 1.0 | ml |
(NH4)2SO4 | 0.5 | g |
Sodium glutamate | 0.5 | g |
Sodium succinate | 0.5 | g |
Sodium acetate | 0.5 | g |
Yeast extract (BD-Difco) | 0.5 | g |
Casamino acids (BD-Difco) | 0.5 | g |
Na2S2O3 | 0.5 | g |
Distilled water | 1.0 | L |
EDTA·3Na | 4.12 | g |
FeSO4·H2O | 1.11 | g |
MgSO4·7H2O | 24.65 | g |
CaCl2·2H2O | 2.94 | g |
NaCl | 23.4 | g |
Trace elements solution (see below) | 10.0 | ml |
Distilled water | 1.0 | L |
KH2PO4 | 75.0 | g |
K2HPO4 | 78.0 | g |
Distilled water | 1.0 | L |
Nicotinic acid | 100.0 | mg |
Thiamine·HCl | 100.0 | mg |
Biotin | 5.0 | mg |
p-Aminobenzoic acid | 50.0 | mg |
Vitamin B12 | 1.0 | mg |
Calcium pantothenate | 50.0 | mg |
Pyridoxine·HCl | 50.0 | mg |
Folic acid | 50.0 | mg |
EDTA·3Na | 200.0 | mg |
Distilled water | 100.0 | ml |
MnSO4·xH2O | 5.58 | g |
ZnSO4·7H2O | 1.44 | g |
Co(NO3)2·6H2O | 1.46 | g |
CuSO4·5H2O | 1.26 | g |
Na2MoO4·2H2O | 1.21 | g |
H3BO3 | 1.55 | g |
EDTA·3Na | 20.6 | g |
Distilled water | 500.0 | ml |