Medium data

Search for medium no. = [303].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

303   PE MEDIUM

Basal salts solution (see below)  5.0     ml
Phosphate solution (see below)  5.0     ml
Vitamin solution (see below)  1.0     ml
(NH4)2SO4  0.5     g
Sodium glutamate  0.5     g
Sodium succinate  0.5     g
Sodium acetate  0.5     g
Yeast extract (BD-Difco)  0.5     g
Casamino acids (BD-Difco)  0.5     g
Na2S2O3  0.5     g
Distilled water  1.0     L
Adjust pH to 7.5.
Basal salts solution:
EDTA·3Na  4.12    g
FeSO4·H2O  1.11    g
MgSO4·7H2O  24.65    g
CaCl2·2H2O  2.94    g
NaCl  23.4     g
Trace elements solution (see below)  10.0     ml
Distilled water  1.0     L
Phosphate solution:
KH2PO4  75.0     g
K2HPO4  78.0     g
Distilled water  1.0     L
Vitamin solution:
Nicotinic acid  100.0     mg
Thiamine·HCl  100.0     mg
Biotin  5.0     mg
p-Aminobenzoic acid  50.0     mg
Vitamin B12  1.0     mg
Calcium pantothenate  50.0     mg
Pyridoxine·HCl  50.0     mg
Folic acid  50.0     mg
EDTA·3Na  200.0     mg
Distilled water  100.0     ml
Trace elements solution:
MnSO4·xH2O  5.58    g
ZnSO4·7H2O  1.44    g
Co(NO3)2·6H2O  1.46    g
CuSO4·5H2O  1.26    g
Na2MoO4·2H2O  1.21    g
H3BO3  1.55    g
EDTA·3Na  20.6     g
Distilled water  500.0     ml



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