Medium data
Search for medium no. = [300].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
300 PETROTOGA MEDIUM
NaCl
30.0
g
MgCl
2
·6H
2
O
0.5
g
CaCl
2
·2H
2
O
0.1
g
NH
4
Cl
1.0
g
KCl
0.2
g
HEPES
2.6
g
Yeast extract (BD-Difco)
1.5
g
Bacto peptone (BD-Difco)
1.5
g
Tryptone (BD-Difco)
1.5
g
KH
2
PO
4
0.3
g
Maltose monohydrate
3.6
g
Distilled water
1.0
L
Adjust pH to 7.5. Fill the gas phase with N
2
.
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