Medium data
Search for medium no. = [299].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
299 RC+ MEDIUM
| NH4Cl | 1.0 | g |
| MgCl2·6H2O | 0.2 | g |
| CaCl2·2H2O | 0.1 | g |
| KCl | 0.1 | g |
| NaCl | 30.0 | g |
| Sodium acetate | 0.83 | g |
| MES | 2.13 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Trypticase peptone (BD-BBL) | 5.0 | g |
| K2HPO4 | 0.3 | g |
| KH2PO4 | 0.3 | g |
| Resazurin | 1.0 | mg |
| L-Cystine | 12.0 | g |
| Na2S·9H2O | 0.5 | g |
| Maltose monohydrate | 7.2 | g |
| Distilled water | 1.0 | L |
Mix ingredients, except Na2S·9H2O and L-cystine, and adjust pH to 6.0 with H2SO4. Fill the gas phase with N2, seal with butyl rubber stopper, and autoclave. Separately autoclave Na2S·9H2O and L-cystine as 5% and 12% solutions, respectively, under a N2 atmosphere. Prior to inoculation, add the sterile Na2S·9H2O and cystine solutions, and readjust pH to 6.0, if necessary. Pressurize the inoculated bottles to 300 kPa N2.
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