Medium data

Search for medium no. = [299].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

299   RC+ MEDIUM

NH4Cl  1.0     g
MgCl2·6H2O  0.2     g
CaCl2·2H2O  0.1     g
KCl  0.1     g
NaCl  30.0     g
Sodium acetate  0.83    g
MES  2.13    g
Yeast extract (BD-Difco)  5.0     g
Trypticase peptone (BD-BBL)  5.0     g
K2HPO4  0.3     g
KH2PO4  0.3     g
Resazurin  1.0     mg
L-Cystine  12.0     g
Na2S·9H2O  0.5     g
Maltose monohydrate  7.2     g
Distilled water  1.0     L
Mix ingredients, except Na2S·9H2O and L-cystine, and adjust pH to 6.0 with H2SO4. Fill the gas phase with N2, seal with butyl rubber stopper, and autoclave. Separately autoclave Na2S·9H2O and L-cystine as 5% and 12% solutions, respectively, under a N2 atmosphere. Prior to inoculation, add the sterile Na2S·9H2O and cystine solutions, and readjust pH to 6.0, if necessary. Pressurize the inoculated bottles to 300 kPa N2.