Medium data
Search for medium no. = [298].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
298 HALOSIMPLEX MEDIUM
| Glycerol | 2.5 | g |
| Sodium pyruvate | 2.5 | g |
| K2HPO4 | 0.5 | g |
| (NH4)2SO4 | 1.0 | g |
| KCl | 4.0 | g |
| NaCl | 200.0 | g |
| MgSO4·7H2O | 20.0 | g |
| Trace metal solution (see below) | 2.0 | ml |
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.15.
For solid agar slopes or plates, add 20.0 g Bacto agar (BD-Difco).
Trace metal solution:
| ZnSO4·7H2O | 0.1 | g |
| MnCl2·4H2O | 0.03 | g |
| H3BO3 | 0.3 | g |
| CoCl2·6H2O | 0.2 | g |
| CuSO4·5H2O | 0.01 | g |
| NiCl2·6H2O | 0.02 | g |
| Na2MoO4·2H2O | 0.03 | g |
| Distilled water | 100.0 | ml |
Adjust pH to 3.0 - 4.0 with HCl.
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