Medium data

Search for medium no. = [295].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

295   CELLULOMONAS FERMENTANS MEDIUM

K2HPO4  2.21    g
KH2PO4  1.5     g
(NH4)2SO4  1.3     g
MgCl2·6H2O  0.1     g
CaCl2·2H2O  0.02    g
FeSO4·7H2O  1.25    mg
Yeast extract  5.0     g
NaHCO3  0.8     g
Distilled water  950  ml
Adjust pH to 7.4. Separately sterilize 50 ml of 10% cellobiose solution, and add to the medium aseptically.