Medium data
Search for medium no. = [292].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
292 LACTOBACILLUS PONTIS MEDIUM
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.5.
After autoclaving, cool to 50C and add aseptically 18 ml Maltose-hemin solution.
Maltose-hemin solution:
| Maltose | 15.0 | g |
| Hemin solution (see below) | 50.0 | ml |
| L-Cysteine·HCl·H2O | 5.0 | g |
| Distilled water | 100.0 | ml |
Filter-sterilize.
Hemin solution:
| Hemin chloride | 0.1 | g |
| Triethanolamine | 4.0 | ml |
| Distilled water | 96.0 | ml |
After filter-sterilizing, store at 4C.
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