Medium data

Search for medium no. = [292].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

292   LACTOBACILLUS PONTIS MEDIUM

Lactobacilli MRS broth (BD-Difco)  55.0     g
Distilled water  1.0     L
Adjust pH to 6.5.
After autoclaving, cool to 50C and add aseptically 18 ml Maltose-hemin solution.
Maltose-hemin solution:
Maltose  15.0     g
Hemin solution (see below)  50.0     ml
L-Cysteine·HCl·H2O  5.0     g
Distilled water  100.0     ml
Filter-sterilize.
Hemin solution:
Hemin chloride  0.1     g
Triethanolamine  4.0     ml
Distilled water  96.0     ml
After filter-sterilizing, store at 4C.