Medium data

Search for medium no. = [287].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

287   WT1 MEDIUM

NaCl  1.5     g
MgCl2·6H2O  10.8     g
Na2SO4  4.0     g
KCl  0.7     g
NaHCO3  0.2     g
KBr  0.1     g
H3BO3  0.03    g
CaCl2·2H2O  1.5     g
SrCl2·6H2O  0.025   g
Tryptone (BD-Difco)  5.0     g
Yeast extract (BD-Difco)  1.0     g
Sulfur (powder)  10.0     g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients, except sulfur and Na2S·9H2O, and adjust pH to 6.8-7.0. Autoclave the medium under a N2 atmosphere. Neutralize Na2S·9H2O as a 5% solution, and autoclave under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Under a N2 stream, mix the medium and sulfur in culture vessels and seal with butyl rubber stoppers. Prior to inoculation, add the Na2S·9H2O solution to the medium aseptically and anaerobically.

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