Medium data
Search for medium no. = [278].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
278 NAM AGAR
| Trypticase soy agar (BD-BBL) | 40.0 | g |
| 5% defibrinated sheep blood | 50.0 | ml |
| Distilled water | 1.0 | L |
After preparing the Trypticase soy agar plate with 5%sheep blood, spreading the subculture on the agar surface and place one NAM disk aseptically. Incubate the agar plate under a H2-CO2-N2 (1:1:8) gas mixture. To prepare the NAM disk, impregnate with 20 µl filter-sterilized 1.5% NAM (N-acetylmuramic acid, Sigma) aqueous solution to a sterile paper disk (0.64 cm in diameter). Stored the air-dried disk at room temperature.
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