Medium data

Search for medium no. = [278].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

278   NAM AGAR

Trypticase soy agar (BD-BBL)  40.0     g
5% defibrinated sheep blood  50.0     ml
Distilled water  1.0     L
After preparing the Trypticase soy agar plate with 5%sheep blood, spreading the subculture on the agar surface and place one NAM disk aseptically. Incubate the agar plate under a H2-CO2-N2 (1:1:8) gas mixture. To prepare the NAM disk, impregnate with 20 µl filter-sterilized 1.5% NAM (N-acetylmuramic acid, Sigma) aqueous solution to a sterile paper disk (0.64 cm in diameter). Stored the air-dried disk at room temperature.