Medium data

Search for medium no. = [275].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

275   MODIFIED CM+YE MEDIUM (B)

Casamino acids, vitamin assay (BD-Difco)  7.5     g
Yeast extract (BD-Difco)  10.0     g
MgSO4·7H2O  10.0     g
Trisodium citrate·2H2O  3.0     g
KCl  2.0     g
NaCl  100.0     g
Fe2+ solution (see below)  1.0     ml
Agar  15.0     g
Distilled water  1.0     L
Adjust pH to 7.4.
Fe2+ solution:
FeSO4·7H2O  4.98    g
Distilled water  100.0     ml