Medium data

Search for medium no. = [267].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

267   AN1 MEDIUM

Trypticase peptone (BD-BBL)  10.0     g
K2HPO4  1.5     g
NaCl  2.5     g
Sodium thioglycolate  1.0     g
Sulfur (powder)  8.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients, except sodium thioglycolate and sulfur, and adjust pH to 7.3. Autoclave the medium under a N2 atmosphere. Separately autoclave sodium thioglycolate as 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Under a N2 stream, mix the medium and sulfur in culture vessels and seal with butyl rubber stoppers. Prior to inoculation, add the thioglycolate solution to the medium anaerobically.