Medium data
Search for medium no. = [267].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
267 AN1 MEDIUM
| Trypticase peptone (BD-BBL) | 10.0 | g |
| K2HPO4 | 1.5 | g |
| NaCl | 2.5 | g |
| Sodium thioglycolate | 1.0 | g |
| Sulfur (powder) | 8.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients, except sodium thioglycolate and sulfur, and adjust pH to 7.3. Autoclave the medium under a N2 atmosphere. Separately autoclave sodium thioglycolate as 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Under a N2 stream, mix the medium and sulfur in culture vessels and seal with butyl rubber stoppers. Prior to inoculation, add the thioglycolate solution to the medium anaerobically.
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