Medium data
Search for medium no. = [264].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
264 T2U MEDIUM
Polypepton (Nihon Pharm. Co.) | 10.0 | g |
Beef extract (BD-Difco) | 5.0 | g |
Yeast extract (BD-Difco) | 2.0 | g |
Glucose | 10.0 | g |
Uracil | 100.0 | mg |
Agar | 15.0 | g |
Distilled water | 1.0 | L |
Adjust pH to 7.0-7.2 with NaOH.
Aseptically add 1 ml of erythromycin solution (10 mg/ml 50% ethanol) to the medium autoclaved.
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