Medium data
Search for medium no. = [252].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
252 SULFOPHOBOCOCCUS MEDIUM
| Yeast extract (BD-Difco) | 1.0 | g |
| Bovine serum albumin | 1.0 | g |
| FeSO4·7H2O | 50.0 | mg |
| EDTA·2Na | 32.0 | mg |
| CaCl2·2H2O | 66.0 | mg |
| MgSO4·7H2O | 31.0 | mg |
| KCl | 31.0 | mg |
| ZnCl2 | 2.1 | mg |
| MnSO4·xH2O | 2.3 | mg |
| Na2B4O7·10H2O | 1.8 | mg |
| Glycine | 1.5 | g |
| Na2CO3 | 230.0 | mg |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components except yeast extract, bovine serum albumin and Na2S·9H2O. Bring to a boil for 5-10 sec and flush with N2. Adjust pH to 7.6 at 80-90C. After cooling to room temperature, dispense the medium into serum bottles under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave yeast extract as a 10% solution and neutralized Na2S·9H2O as a 5% solution under a N2 atmosphere. Filter-sterilize bovine serum albumin as a 10% (w/v) solution under a N2 atmosphere. Before inoculation, aseptically and anaerobically add the yeast extract, bovine serum albumin and neutralized Na2S·9H2O solutions.
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