246 PPES-II AGAR MEDIUM
| Peptone | 2.0 | g |
| Proteose peptone No. 3 (BD-Difco) | 1.0 | g |
| Soytone | 1.0 | g |
| Yeast extract | 1.0 | g |
| EDTA·Fe(III) | 0.1 | g |
| Agar | 15.0 | g |
| Artificial seawater (see below) | 1.0 | L |
| NaCl | 30.0 | g |
| KCl | 0.7 | g |
| MgCl2·6H2O | 10.8 | g |
| MgSO4·7H2O | 5.4 | g |
| CaCl2·2H2O | 1.0 | g |
| Distilled water | 1.0 | L |