Medium data

Search for medium no. = [239].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

239

L-Asparagine	`1.0`	g
Glycerol	`2.0`	g
K₂HPO₄	`1.0`	g
Polypepton (Nihon Pharm. Co.)	`30.0`	g
Trace salts solution (see below)	`1.0`	ml
Vitamin B solution (see below)	`10.0`	ml
Agar	`15.0`	g

Add components, except vitamin B solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.2. After autoclaving, aseptically add filter-sterilized vitamin B solution.
Trace salts solution:

FeSO₄·7H₂O	`0.1`	g
MnCl₂·4H₂O	`0.1`	g
ZnSO₄·7H₂O	`0.1`	g
Distilled water	`100.0`	ml

Vitamin B solution:

Thiamine·HCl	`10.0`	mg
Riboflavin	`10.0`	mg
Nicotinate	`10.0`	mg
Pyridoxine·HCl	`10.0`	mg
myo-Inositol	`10.0`	mg
Calcium pantothenate	`10.0`	mg
p-Aminobenzoic acid	`10.0`	mg
D-Biotin	`5.0`	mg
Distilled water	`200.0`	ml