Medium data

Search for medium no. = [220].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

220 SC MEDIUM

Corn meal agar (BD-Difco)	`17.0`	g
Phytone peptone (BD-BBL)	`8.0`	g
K₂HPO₄	`1.0`	g
KH₂PO₄	`1.0`	g
MgSO₄·7H₂O	`0.2`	g
Hemin (0.1% in 0.05 N NaOH)	`15.0`	ml
20% Bovine serum albumin, fraction V	`10.0`	ml
50% Glucose	`1.0`	ml
10% Cysteine	`10.0`	ml
Distilled water	`1.0`	L

Adjust pH to 6.6.
Separately sterilize bovine serum albumin, glucose and cysteine solutions by filtration, and aseptically add to the medium.