Medium data

Search for medium no. = [21].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

21   LACTOBACILLUS MEDIUM III

Casein peptone, tryptic digest  10.0     g
Yeast extract (BD-Difco)  5.0     g
Beef extract (BD-Difco)  2.0     g
Glucose  20.0     g
KH2PO4  0.5     g
K2HPO4  0.5     g
FeSO4·7H2O  10.0     mg
MgSO4·7H2O  0.2     g
MnSO4·xH2O  7.5     mg
Tween 80  1.0     ml
Ethanol  40.0     ml
Sodium acetate  20.0     g
DL-Mevalonic acid  30.0     mg
Agar  15.0     g
Distilled water  1.0     L
Adjust pH to 5.2.