Medium data

Search for medium no. = [196].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

196   THERMOPROTEUS TENAX MEDIUM

(NH4)2SO4  0.264   g
FeSO4·7H2O  0.556   g
MgSO4·7H2O  0.492   g
CaSO4·2H2O  0.344   g
KH2PO3  0.014   g
NaF  0.84    mg
MnCl2·4H2O  0.18    mg
Na2B4O7·10H2O  0.45    mg
ZnSO4·7H2O  22.0     µg
CuCl2·2H2O  5.0     µg
Na2MoO7·2H2O  3.0     µg
CoSO4·7H2O  1.0     µg
Yeast extract (BD-Difco)  0.2     g
Soluble starch  5.0     g
Sulfur (powder)  10.0     g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients, except yeast extract, starch, sulfur and Na2S·9H2O, and filter-sterilize while gassing with N2. Autoclave yeast extract and starch as 10% solutions and Na2S·9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract and starch solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4.