Medium data
Search for medium no. = [182].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
182 MODIFIED HORIKOSHI-I MEDIUM
| Glucose | 10.0 | g |
| Hipolypepton (FUJIFILM Wako) | 5.0 | g |
| Yeast extract | 5.0 | g |
| NaCl | 10.0 | g |
| K2HPO4 | 1.0 | g |
| MgSO4·7H2O | 0.2 | g |
| Agar | 15.0 | g |
| Distilled water | 900.0 | ml |
After autoclaving, aseptically add 100 ml of a sterile 10% NaHCO3 solution to the medium. Check final pH to be about 9.0.
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