Medium data

Search for medium no. = [181].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

181   HORIKOSHI-I MEDIUM

Glucose  10.0     g
Polypepton (Nihon Pharm. Co.)  5.0     g
Yeast extract  5.0     g
K2HPO4  1.0     g
MgSO4·7H2O  0.2     g
Agar  15.0     g
Distilled water  900.0     ml
After autoclaving, aseptically add 100 ml of a sterile 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.