Medium data

Search for medium no. = [180].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

180   THERMOPLASMA MEDIUM

(NH4)2SO4  0.2     g
KH2PO4  3.0     g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.25    g
Yeast extract (BD-Difco)  1.0     g
Glucose  10.0     g
Distilled water  1.0     L
Adjust pH to 2.0 with 10 N H2SO4. Separately autoclave yeast extract and glucose as 10% solutions, and add aseptically to the medium.