Medium data
Search for medium no. = [18].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
18 LACTIC ACID BACTERIA BROTH
| Trypticase peptone (BD-BBL) | 10.0 | g |
| Yeast autolysate | 5.0 | g |
| Sodium acetate | 12.0 | g |
| Glucose | 10.0 | g |
| Solution A (see below) | 5.0 | ml |
| Solution B (see below) | 5.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 5.1-5.3.
Solution A:
| K2HPO4 | 10.0 | g |
| KH2PO4 | 10.0 | g |
| Distilled water | 100.0 | ml |
Solution B:
| MgSO4·7H2O | 4.0 | g |
| NaCl | 0.2 | g |
| FeSO4·7H2O | 0.2 | g |
| MnSO4·xH2O | 0.2 | g |
| Distilled water | 100.0 | ml |
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