Medium data

Search for medium no. = [176].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

176   ACIDIANUS BRIERLEYI MEDIUM

(NH4)2SO4  3.0     g
KH2PO4  0.5     g
MgSO4·7H2O  0.5     g
KCl  0.1     g
Ca(NO3)2  0.01    g
Yeast extract (BD-Difco)  0.2     g
Sulfur (powder)  10.0     g
Distilled water  1.0     L
Adjust pH to 2.0 with 10 N H2SO4. Autoclave yeast extract separately. Steam sulfur for 3 hr on each of 3 successive days.