Medium data
Search for medium no. = [176].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
176 ACIDIANUS BRIERLEYI MEDIUM
| (NH4)2SO4 | 3.0 | g |
| KH2PO4 | 0.5 | g |
| MgSO4·7H2O | 0.5 | g |
| KCl | 0.1 | g |
| Ca(NO3)2 | 0.01 | g |
| Yeast extract (BD-Difco) | 0.2 | g |
| Sulfur (powder) | 10.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 2.0 with 10 N H2SO4. Autoclave yeast extract separately. Steam sulfur for 3 hr on each of 3 successive days.
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