Medium data
Search for medium no. = [173].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
173 BARBOUR-SFOENNER-KELLY (BSK) II MEDIUM
Solution A:
| CMRL 1066 with glutamate (Sigma) | 4.95 | g |
| Bovine serum albumin, Fraction V (Wako) | 25.0 | g |
| HEPES (Sigma) | 3.0 | g |
| Trisodium citrate | 0.35 | g |
| Glucose | 2.5 | g |
| Sodium pyruvate (Sigma) | 0.4 | g |
| N-Acetyl-D-glucosamine (Sigma) | 0.2 | g |
| NaHCO3 (Sigma) | 1.1 | g |
| TC Yeastolate (BD-Difco) | 1.0 | g |
| Distilled water | 500.0 | ml |
Solution B:
| Neopeptone (BD-Difco) | 2.5 | mg |
| Gelatin | 7.0 | g |
| Distilled water | 100.0 | ml |
Adjust pH to 7.6 with 1 N NaOH and sterilize by filtration through a 0.22 µm filter (Millipore). Autoclave Solution B, then mix Solutions A and B, and add 40 ml of heat-inactivated (56C, 45 min) sterile rabbit serum (Pel-Freez Biologicals).
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