Medium data
Search for medium no. = [170].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
170 ARTIFICIAL DEEP LAKE VITAMIN MEDIUM
| NaCl | 180.0 | g |
| MgCl2·6H2O | 75.0 | g |
| MgSO4·7H2O | 7.4 | g |
| KCl | 7.4 | g |
| CaCl2·2H2O | 1.0 | g |
| Sodium succinate | 10.0 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| Vitamin solution (see below) | 10.0 | ml |
Add components, except vitamin solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.4. For preparation of solid medium, add 20.0 g/L Noble Agar (BD-Difco). Autoclave. After cooling to 50C, aseptically add the following vitamin solution (filter-sterilized):
Vitamin solution:
| Biotin | 0.1 | g |
| Vitamin B12 | 0.1 | g |
| Thiamine·HCl | 0.1 | g |
| Distilled water | 1.0 | L |
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