Medium data

Search for medium no. = [170].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

170   ARTIFICIAL DEEP LAKE VITAMIN MEDIUM

NaCl  180.0     g
MgCl2·6H2O  75.0     g
MgSO4·7H2O  7.4     g
KCl  7.4     g
CaCl2·2H2O  1.0     g
Sodium succinate  10.0     g
Yeast extract (BD-Difco)  1.0     g
Vitamin solution (see below)  10.0     ml
Agar noble (BD-Difco)  20.0     g
Add components except Vitamin solution to distilled water and bring volume to 990 ml. Adjust pH to 7.4. After autoclaving, aseptically add filter-sterilized Vitamin solution.
Vitamin solution:
Biotin  0.1     g
Vitamin B12  0.1     g
Thiamine·HCl  0.1     g
Distilled water  1.0     L