Medium data

Search for medium no. = [170].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  180.0     g
MgCl2·6H2O  75.0     g
MgSO4·7H2O  7.4     g
KCl  7.4     g
CaCl2·2H2O  1.0     g
Sodium succinate  10.0     g
Yeast extract (BD-Difco)  1.0     g
Vitamin solution (see below)  10.0     ml
Add components, except vitamin solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.4. For preparation of solid medium, add 20.0 g/L Noble Agar (BD-Difco). Autoclave. After cooling to 50C, aseptically add the following vitamin solution (filter-sterilized):
Vitamin solution:
Biotin  0.1     g
Vitamin B12  0.1     g
Thiamine·HCl  0.1     g
Distilled water  1.0     L

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