Medium data

Search for medium no. = [166].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

166   

Casamino acids (BD-Difco)  15.0     g
Trisodium citrate  3.0     g
Glutamic acid  2.5     g
MgSO4·7H2O  2.5     g
KCl  2.0     g
NaCl  250.0     g
Agar  20.0     g
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0. After autoclaving, readjust pH to 8.5 with a sterile 5% Na2CO3 solution.