Medium data
Search for medium no. = [16].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
16 LACTOBACILLUS MEDIUM II
| Trypticase peptone (BD-BBL) | 10.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Tryptose (BD-Difco) | 3.0 | g |
| KH2PO4 | 3.0 | g |
| K2HPO4 | 3.0 | g |
| Salt solution R (see below) | 5.0 | ml |
| Tween 80 | 1.0 | ml |
| Sodium acetate | 1.0 | g |
| L-Cysteine·HCl·H2O | 0.2 | g |
| Agar | 20.0 | g |
| Glucose | 5.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.3.
Salt solution R:
| MgSO4·7H2O | 11.5 | g |
| FeSO4·7H2O | 0.68 | g |
| MnSO4·xH2O | 2.4 | g |
| Distilled water | 1.0 | L |
Store at 4C.
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved