Medium data

Search for medium no. = [16].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

16   LACTOBACILLUS MEDIUM II

Trypticase peptone (BD-BBL)  10.0     g
Yeast extract (BD-Difco)  5.0     g
Tryptose (BD-Difco)  3.0     g
KH2PO4  3.0     g
K2HPO4  3.0     g
Salt solution R (see below)  5.0     ml
Tween 80  1.0     ml
Sodium acetate  1.0     g
L-Cysteine·HCl·H2O  0.2     g
Agar  20.0     g
Glucose  5.0     g
Distilled water  1.0     L
Adjust pH to 6.3.
Salt solution R:
MgSO4·7H2O  11.5     g
FeSO4·7H2O  0.68    g
MnSO4·xH2O  2.4     g
Distilled water  1.0     L
Store at 4C.



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