Medium data

Search for medium no. = [1475].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1475   DESULFOBACTER MEDIUM WITH HORSE SERUM

Na2SO4       3.0     g
KH2PO4       0.2     g
NH4Cl        0.3     g
NaCl        21.0     g
MgCl2·6H2O        3.0     g
KCl           0.5     g
CaCl2·2H2O        0.15    g
Tryptone (Difco)       10.0     g
FeCl2 solution (see Medium No. 187)      1.0     ml
Trace element solution (see Medium No. 187)     1.0     ml
Resazurin          1.0     mg
Distilled water       920.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filtersterilized):
Horse Serum (Gibco)*       10.0     ml
Trace vitamins* (see Medium No. 197)       10.0     ml
8% NaHCO3 solution*       50.0     ml
5% Na2S·9H2O solution       8.0     ml
Adjust pH 7.1-7.4, if necessary.



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