Medium data

Search for medium no. = [1468].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1468 FRESHWATER R2A MEDIUM

Yeast extract (BD-Difco)	`0.5`	g
Proteose peptone No. 3 (BD-Difco)	`0.5`	g
Casamino acids (BD-Difco)	`0.5`	g
Glucose	`0.5`	g
KH₂PO₄	`0.5`	g
Soluble starch	`0.5`	g
Sodium pyruvate	`0.5`	g
NH₄Cl	`1.5`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Selenite tungstate solution (see Medium No. 431)	`0.5`	ml
Vitamin solution* (see Medium No. 403)	`1.0`	ml
Thiamine solution* (see Medium No. 403)	`1.0`	ml
Vitamin B12 solution* (see Medium No. 403)	`1.0`	ml
Resazurin	`0.5`	mg
Distilled water	`1.0`	L

Mix components and autoclave under a N₂-CO₂ (80:20, v/v) gas mixture. After cooling, aseptically and anaerobically, add the following solutions from anaerobic stocks (autoclaved and stored under a N₂ atmosphere):

1 M NaHCO₃ solution	`5.0`	ml
1 M Coenzyme M solution	`0.5`	ml
5% Na₂S·9H₂O solution	`6.0`	ml

Adjust pH to 7.0.