Medium data
Search for medium no. = [1462].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1462 METHANOSARCINA T3 MEDIUM
| KCl | 0.34 | g |
| MgCl2·6H2O | 4.0 | g |
| NH4Cl | 0.25 | g |
| CaCl2·2H2O | 0.14 | g |
| K2HPO4 | 0.14 | g |
| NaCl | 9.0 | g |
| Trace mineral solution (see Medium No. 151) | 10.0 | ml |
| Ni-Se-W solution (see below) | 10.0 | ml |
| Fe(NH4)2(SO4)2·6H2O | 2.0 | mg |
| NaHCO3 | 5.0 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 980.0 | ml |
Mix components thoroughly and adjust pH to 6.5, then distribute the medium into culture vessels under a N2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Prior to use, add per liter the following solutions (autoclaved or* filter-sterilized, and stored under a N2 atmosphere):
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
| 2 M trimethylammonium chloride solution* | 35.0 | ml |
| Methanol* | 5.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
Adjust pH to 7.0 if necessary.
Ni-Se-W solution:
| NiCl2·6H2O | 30.0 | mg |
| Na2SeO3·5H2O | 0.3 | mg |
| Na2WO2·2H2O | 0.4 | mg |
| Distilled water | 1.0 | L |
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