Medium data

Search for medium no. = [142].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution A:
(NH4)2SO4  2.0     g
KCl  0.1     g
K2HPO4  0.5     g
MgSO4·7H2O  0.5     g
Yeast extract (BD-Difco)  0.1     g
Distilled water  500.0     ml
Adjust pH to 3.5 with H2SO4 solution.
Solution B:
Glucose  1.0     g
Distilled water  500.0     ml
Autoclave Solutions A and B separately, and mix aseptically. For preparation of solid medium, add 15.0 g/L agar in solution B before autoclaving.

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