Medium data
Search for medium no. = [1419].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1419 HALOTHIOBACILLUS LS2 MEDIUM
| KH2PO4 | 0.4 | g |
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.12 | g |
| Modified A5 solution (see below) | 1.0 | ml |
| Distilled water | 1.0 | L |
Mix components, adjust pH to 6.5-7.0, and autoclave. For preparation of solid media, add 15.0 g/L agar and 0.1 g/L Na2SO4 before autoclaving. After cooling, add the following solutions (filter-sterilized):
| 8% NaHCO3 solution | 2.5 | ml |
| 0.1 M M FeCl2 solution | 0.4 | ml |
| 1.0 M NH4Cl solution | 1.0 | ml |
| 1.0 M Sodium thiosulfate solution | 10.0 | ml |
Modified A5 solution:
| H3BO3 | 2.86 | g |
| ZnSO4·7H2O | 0.222 | g |
| Na2MoO4·2H2O | 0.39 | g |
| MnCl2·4H2O | 1.86 | g |
| CuSO4·5H2O | 0.079 | g |
| CoCl2·6H2O | 0.04 | g |
| Distilled water | 1.0 | L |
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