Medium data
Search for medium no. = [14].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
14 EG MEDIUM
| Lab-Lemco powder (Oxoid) | 2.4 | g |
| Proteose peptone No. 3 (BD-Difco) | 10.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Na2HPO4 | 4.0 | g |
| Glucose | 1.5 | g |
| Soluble starch | 0.5 | g |
| L-Cystine | 0.2 | g |
| Agar | 15.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Horse blood | 50.0 | ml |
| Distilled water | 950.0 | ml |
Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.
Copyright © 2025 Microbe Division (JCM) - All Rights Reserved