Medium data
Search for medium no. = [1375].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1375 MODIFIED LOW SALT MEDIUM FOR B11
| NaCl | 9.0 | g |
| MgCl2·6H2O | 3.0 | g |
| KCl | 0.5 | g |
| NH4Cl | 0.3 | g |
| KH2PO4 | 0.2 | g |
| CaCl2·2H2O | 0.15 | g |
| Yeast extract | 1.0 | g |
| Trace element solution (see Medium No. 284) | 2.0 | ml |
| Resazurin | 0.5 | mg |
| Distilled water | 867.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 25.0 | ml |
| 10% Glucose solution | 100.0 | ml |
| Trace vitamins solution* (see Medium No. 284) | 2.0 | ml |
| 5% Na2S·9H2O solution | 6.0 | ml |
Adjust pH to 6.5-7.0, if necessary.
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