Medium data

Search for medium no. = [1327].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1327   GEOBACTER NBAF MEDIUM

KH2PO4  0.42    g
K2HPO4  0.22    g
NH4Cl  0.20    g
KCl  0.38    g
NaCl  0.36    g
CaCl2·2H2O  0.04    g
Fe2(SO4)3·nH2O  0.01    g
MgSO4·7H2O  0.1     g
0.1% Na2SeO4 solution  1.0     ml
NB Mineral elixir solution (see below)  10.0     ml
Distilled water  900.0     ml
Mix components and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture, and add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized).
8% NaHCO3 solution*  20.0     ml
5% Na2CO3 solution*  10.0     ml
Trace vitamins* (see Medium No. 197)  15.0     ml
1 M Sodium acetate solution  15.0     ml
1 M Sodium fumarate solution  40.0     ml
Check final pH to be around 7.0.
NB Mineral elixir solution:
Nitrilotriacetic acid  2.14    g
MnCl2·4H2O  0.1     g
FeSO4·7H2O  0.3     g
CoCl2·6H2O  0.17    vg
ZnSO4·7H2O  0.2     vg
CuCl2·2H2O  0.03    vg
AlK(SO4)2  0.003   vg
H3BO3  0.005   g
Na2MoO4·2H2O  0.09    g
NiSO4·6H2O  0.11    g
Na2WO2·2H2O  0.02    g
Dissolve nitrilotriacetic acid and adjust pH to 8.0-8.5 with NaOH. Then proceed to add minerals.