Medium data
Search for medium no. = [1326].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1326 MODIFIED DESULFOVIBRIO MEDIUM
Solution A:
| K2HPO4 | 0.5 | g |
| KH2PO4 | 0.5 | g |
| NH4CI | 1.0 | g |
| Na2SO4 | 1.0 | g |
| MgSO4·7H2O | 2.0 | g |
| CaCl2·2H2O | 0.1 | g |
| Sodium lactate | 2.0 | g |
| Yeast extract | 1.0 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 980.0 | ml |
SolutionB:
| FeSO4·7H2O | 0.1 | g |
| Distilled water | 10.0 | ml |
SolutionC:
| Sodium thioglycolate | 0.1 | g |
| Ascorbic acid | 0.1 | g |
| Distilled water | 10.0 | ml |
Dissolve ingredients of each solution in the appropriate amount of distilled water. Bring solution A to a boil and cool down to room temperature under a N2 gas stream. Add solutions Band C to the medium, then, adjust pH to 7.3 with NaOH. While swirling the medium, dispense into culture vessels under a N2 gas stream, seal with butyl rubber stoppers and autoclave.
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