Medium data

Search for medium no. = [1321].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1321   9K WITH SULFUR MEDIUM

K2HPO4  0.5     g
(NH4)2SO4  3.0     g
KCl  0.1     g
MgSO4·7H2O  0.5     g
Ca(NO3)2  10.0     mg
Sulfur (powder)  5.0     g
Distilled water  1.0     L
Mix components except sulfur, adjust pH to 2.5 with H2SO4 and autoclave. Separately sterilize sulfur by steaming for 3 hr on each of 3 successive days.