Medium data
Search for medium no. = [1321].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1321 9K WITH SULFUR MEDIUM
| K2HPO4 | 0.5 | g |
| (NH4)2SO4 | 3.0 | g |
| KCl | 0.1 | g |
| MgSO4·7H2O | 0.5 | g |
| Ca(NO3)2 | 10.0 | mg |
| Sulfur (powder) | 5.0 | g |
| Distilled water | 1.0 | L |
Mix components except sulfur, adjust pH to 2.5 with H2SO4 and autoclave. Separately sterilize sulfur by steaming for 3 hr on each of 3 successive days.
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