Medium data

Search for medium no. = [1318].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1318 YCFA MEDIUM WITH 10% RUMEN FLUID

Use Medium No. 1130 with 10% rumen fluid, clarified (see below).
To prepare the clarified rumen fluid, preheat rumen content at 121C for 15 min. Use the supernatant after centrifuging at 25,000 x g for 15 min.