Medium data

Search for medium no. = [1315].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1315   METHANOGEN MS-I MEDIUM

NaCl  0.45    g
NaHCO3  0.2     g
MgSO4·7H2O  0.09    g
KH2PO4  0.225   g
K2HPO4  0.225   g
(NH4)2SO4  0.225   g
CaCl2·2H2O  0.06    g
(NH4)2Ni(SO4)2·6H2O  2.0     mg
FeSO4·7H2O  2.0     mg
Fe2(SO4)3·nH2O  2.0     mg
Wolfe's mineral elixir (see Medium No. 470)  0.1     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly, except NaHCO3, and adjust pH to 7.0. Bring to a boil for a seconds, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solutions (autoclaved or filter-sterilized, and stored under N2):
Trace vitamins* (see Medium No. 197)  10.0     ml
1.0 M Sodium acetate solution  10.0     ml
5% Na2S·9H2O solution  10.0     ml
Pressurize inoculated bottles to 100 KPa H2-CO2 (80:20, v/v).
Comment: For JCM 39178, add total 30.0 ml of 1.0 M sodium acetate solution in the above medium recipe.