Medium data

Search for medium no. = [1305].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1305 HELICOBACTER MEDIUM

Brucella broth (BD-BBL)	`2.8`	g
Distilled water	`80.0`	ml

Autoclave. After cooling to 50C and add aseptically 135 µl of conc. HCl (final conc. 0.05%). Then add aseptically the following solutions.

Fetal bovine serum (Biowest; heat inactivated)	`20.0`	ml
Vitox (Oxoid) (1 vial/10 ml of rehydration fluid)	`2.0`	ml
Skirrow supplement (Oxoid) (1 vial/2 ml of sterile, purified water)	`0.4`	ml
Amphotericin B (5 mg/ml DMSO)	`100.0`	µl

For stable culture, incubate the microorganisms in 5 ml of above-mentioned broth in a polystyrene culture flask (25 cmz² flask, canted neck, vent cap, AS ONE: Cat. No. 2-8589-01) at 37C under a 5% O₂ and 12% CO₂ atmosphere (with shaking at low speed; 50 rpm).