Medium data
Search for medium no. = [1299].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1299 PAENIBACILLUS DC-8A MEDIUM
| K2HPO4 | 2.9 | g |
| Urea | 4.2 | g |
| Yeast extract | 2.0 | g |
| CaCl2·2H2O | 0.01 | g |
| Na2CO3 | 1.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 0.5 | mg |
| Mineral solution (see below) | 0.2 | ml |
| Distilled water | 900.0 | ml |
Mix components thoroughly and adjust pH to 9.0. Distribute the medium into culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere):
| 10% (w/v) Xylose solution | 100.0 | ml |
Mineral solution:
| MgCl2·6H2O | 25.0 | g |
| CaCl2·2H2O | 37.5 | g |
| FeSO4·7H2O | 0.312 | g |
| Distilled water | 1.0 | L |
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