Medium data

Search for medium no. = [1299].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1299   PAENIBACILLUS DC-8A MEDIUM

K2HPO4  2.9     g
Urea  4.2     g
Yeast extract  2.0     g
CaCl2·2H2O  0.01    g
Na2CO3  1.0     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  0.5     mg
Mineral solution (see below)  0.2     ml
Distilled water  900.0     ml
Mix components thoroughly and adjust pH to 9.0. Distribute the medium into culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere):
10% (w/v) Xylose solution  100.0     ml
Mineral solution:
MgCl2·6H2O  25.0     g
CaCl2·2H2O  37.5     g
FeSO4·7H2O  0.312   g
Distilled water  1.0     L



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