Medium data

Search for medium no. = [1295].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution A:
Yeast extract  3.0     g
Peptone  5.0     g
Beef extract  10.0     g
Glucose  5.0     g
L-Cysteine·HCl·H2O  0.5     g
NaCl  5.0     g
Sodium acetate  3.0     g
Soluble starch  1.0     g
Distilled water  900.0     ml
Adjust pH to 6.5-7.0.
Solution B:
Na2CO3  7.0     g
NaHCO3  2.9     g
Distilled water  100.0     ml
Separately autoclave Solutions A and B, and combine after cooling. Cultivate under a static culture condition or a N2 atmosphere. For preparation of solid medium, add 10.0 g gellan gum in Solution A (900 ml). After autoclaving, mix the two solutions while being hot (60 to 70 C) and pour into test tubes or petri dishes.

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