Medium data
Search for medium no. = [1291].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1291 GS2 MEDIUM WITH CELLOBIOSE
| KH2PO4 | 1.5 | g |
| K2HPO4 | 2.9 | g |
| Urea | 2.1 | g |
| MOPS | 10.0 | g |
| Yeast extract | 6.0 | g |
| Trisodium citrate·2H2O | 2.9 | g |
| L-Cysteine·HCl·H2O | 0.25 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.2 with NaOH. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add the following solution (autoclaved):
| GS2 salt solution (see below) | 10.0 | ml |
| 0.2 M Cellobiose solution | 8.0 | ml |
GS2 salt solution:
| MgCl2·6H2O | 100.0 | g |
| CaCl2·2H2O | 15.0 | g |
| 1.25% FeSO4 solution | 0.1 | ml |
| Distilled water | 1.0 | L |
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